In conventional chromatography, separation of similar compounds can be improved by gradient elution.
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In gradient elution, the elution order may change as the dimensions or flow rate change.
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Additionally, they were able to tune the retention factor for the analytes through isocratic temperature gradient elution.
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A separation in which the mobile phase composition is changed during the separation process is described as a " gradient elution ".
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A chloroform-ethanol gradient elution High-performance liquid chromatography system was used to extract the iridoid glycosides campenoside and 5-hydroxycampenoside.
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Gradient elution uses this effect by automatically reducing the polarity and the surface tension of the aqueous mobile phase during the course of the analysis.
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Gradient elution decreases the retention of the later-eluting components so that they elute faster, giving narrower ( and taller ) peaks for most components.
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Resolution in elution chromatography is generally better when peaks are strongly retained, but conditions that give good resolution of early peaks lead to long run-times and excessive broadening of later peaks unless gradient elution is employed.
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This enabled them to make a number of major technological improvements, including the introduction of fiber suppressors ( 1981 ), a metal-free system eliminating sources of contamination and corrosion ( 1981 ), integrated workstation and process analysis capabilities ( 1984 ), suppressors for gradient elution ( 1986 ) and accelerated solvent extraction ( 1995 ).
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